Compartmental organization of the olfactory bulb glomerulus.

摘要: Olfactory receptor cell (ORC) axons terminate in the olfactory bulb glomerular neuropil, where they synapse with dendrites of mitral, tufted, and periglomerular neurons. We investigated organization neuropil by using antibodies to both single- double-label constituents for analyses confocal microscopy. Electron microscopy (EM) was employed assess distribution synaptic appositions within glomerulus. Adult Sprague-Dawley rats were processed immunocytochemistry marker protein (OMP), synaptophysin, synapsin 1, glial fibrillary acidic (GFAP), and/or microtubule-associated 2 (MAP2). Equivalent transmission EM. Double labeling OMP MAP2 revealed two distinctive subcompartments glomeruli: an axonal compartment containing predominately primary afferent individual dendritic inserts a complementary that excluded axons. Areas not occupied or immunoreactivity either immunoreactive GFAP, indicating process, blood vessels. Synaptophysin 1 also showed differential strongly colocalized OMP, whereas associated most MAP2. Reconstructions glomeruli from EM montages interdigitating subcompartments. The composed primarily ORC processes small groups interspersed. Dendritic processes. Primary axodendritic local-circuit dendrodendritic synapses segregated glomerulus into subcompartments, respectively. results support hypothesis subcompartmental glomeruli. J. Comp. Neurol. 407:261–274, 1999. © 1999 Wiley-Liss, Inc.