Intracellular dynamics and transcriptional regulations in plant zygotes: a case study of Arabidopsis

摘要: Recent understandings ofArabidopsiszygote. Body axis formation is essential for the proper development of multicellular organisms. The apical-basal in Arabidopsis thaliana determined by asymmetric division zygote, following its cellular polarization. However, regulatory mechanism zygote polarization unclear due to technical issues. located deep seed (ovule) flowers, which prevents living dynamics zygotes from being observed. In addition, elucidation molecular pathways conventional forward genetic screens was not enough because high gene redundancy early development. Here, we present a review introducing two new methods, have been developed overcome these problems. Method 1: two-photon live-cell imaging method provides system visualize intracellular structures zygotes, such as cytoskeletons and vacuoles. Microtubules form transverse rings control elongation, while vacuoles dynamically change their shapes along longitudinal actin filaments support polar nuclear migration. 2: transcriptome uses isolated egg cells reveal expression profiles before after fertilization. This approach revealed that de novo transcription occurs extensively immediately Moreover, inhibition shown sufficiently block zygotic division, thus indicating strong possibility yet unidentified regulators can be found using this approach. These strategies will help further our understanding fundamental principles regarding plant bodies unicellular zygotes.