Rufloxacin-induced photosensitization in yeast.

摘要: The fluoroquinolone Rufloxacin (RFX) is active as specific inhibitor of bacterial gyrase. adverse effects the photosensitization induced by fluoroquinolones are well known. A predominant type II photosensitizing activity has already been demonstrated on simpler models (free nucleosides, calf thymus DNA), whereas a cooperative mechanism was corroborated more complex ones (plasmid and fibroblast). purpose this study to examine drug photocytoxicity in another cellular model, wild-type eukaryotic fast-growing microorganism whose cultivation cheap easily managed, Saccharomyces cerevisiae. This work represents first report potential photogenotoxicity Rufloxacin. Particular emphasis given DNA modifications caused yeast formation photomediated toxic species, such hydrogen peroxide formaldehyde. Drug phototoxicity evaluated measuring fragmentation (single/double strand breaks) using single cell gel electrophoresis assay 8-OH-dGuo, photooxidation biomarker, HPLC-ECD. Cellular sensitivity also assessed viability test. extra- intracellular RFX concentration (as its main photoproduct) verified HPLC-MS, cytotoxic species were colorimetric assays. results confirm agreement with those previously obtained human fibroblast used recently, provide clear link between overall phototoxicity.