Use of global amino acid replacements to define the requirements for MHC binding and T cell recognition of moth cytochrome c (93-103).

摘要: Substitution with all naturally occurring L-amino acids at each of 11 residues the IEk-restricted month cytochrome c (93-103) epitope has allowed us to analyze requirements for MHC binding and T cell recognition a level definition not previously possible. Substitutions only three positions systematically affect others appear be major TCR contacts. Interestingly, changing involved in can ablate without altering association. Additionally, residue identity two that do critical binding, nor specific contact, nonetheless dramatically responses. This suggests peptides differing slightly sequence have significantly altered conformations within class II groove. We also developed simple scoring program uses data quantitate how well given peptide fits MCC motif. All strongly immunogenic epitopes score highly (> or = 0.70, where 1.0 is perfect concordance), 3% potential nonameric main protein databases scores greater than 0.70. indicates global amino acid replacement approach using single an efficient means deriving motifs molecule, should aid identification novel epitopes.