High‐affinity immunoglobulin E receptor (FcεRI)‐bearing eosinophils, mast cells, macrophages and Langerhans’ cells in allergen‐induced late‐phase cutaneous reactions in atopic subjects
作者： YING , BARATA , MENG , GRANT , BARKANS
DOI: 10.1046/J.1365-2567.1998.00418.X
关键词:
摘要: We have used in situ hybridization (ISH) and immunohistochemistry (IHC) to investigate the kinetics of expression for Fc epsilon RI mRNA (alpha-, beta- gamma-chains), alpha-chain protein product, as well phenotype mRNA- or protein-positive cells allergen-induced late-phase skin reactions atopic subjects. Compared with diluent controls, there were significant increases total number mRNA+ alpha-, gamma-chains at all time-points (6, 24 48 hr) after allergen challenge (P < 0.01). By double IHC/ISH gamma-chain macrophages, eosinophils, mast CD1a+ also observed 0.05). The distribution subunit beta-, gamma-chain) co-localization was CD68+ macrophages (42-47%), EG2+ eosinophils (33-39%), tryptase+ (5-11%) Langerhans' (2-4%). Using single IHC, protein+ 0.01) hr challenge. Double IHC showed that RI+ (33%), (36%), (20%), (4%) unidentified (7%), 24-hr allergen-challenged sites. These observations suggest cutaneous reaction man is associated up-regulation on cells.
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