Mechanisms Involved in Resveratrol‐Induced Apoptosis and Cell Cycle Arrest in Prostate Cancer—Derived Cell Lines

摘要: Resveratrol is a polyphenol found at high concentrations in grapes and red wine with reported anticarcinogenic effects. We studied the molecular mechanism of resveratrol-induced apoptosis proliferation arrest prostate derived cells PZ-HPV-7 (nontumorigenic line), LNCaP (androgen-sensitive cancer PC-3 (androgen-insensitive line). Apoptosis cell cycle distribution were evaluated by flow cytometry MTT assay direct counting. Caspases, bax, bcl-2, cyclins, Cdks, p53, p21, p27 measured Western blot kinase activities cyclin/Cdk complexes immunoprecipitation followed assays appropriate substrates. induced decrease rates an increase lines dose- time-dependent manner. These effects coincident accumulation G0/G1 phase. In PC-3, resveratrol was mediated activation caspases 9 3 change ratio bax/bcl-2. Expressions cyclin D1, E, Cdk4 as well D1/Cdk4 activity reduced only cells. contrast, B Cdk1 expression B/Cdk1 decreased both presence resveratrol. However, modulator proteins increased probably result observed disruption control. addition, specific differences between suggest that acts through different mechanisms upon androgen or estrogen receptor status.