Competitive Peptide Adsorption to Nitrocellulose Covered Backing Material in Cf-252-Plasma Desorption Mass Spectrometry
作者： G. Allmaier , E. Pittenauer , E. R. Schmid
DOI: 10.1007/978-1-4684-7926-3_5
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摘要: Cf-252-Plasma desorption (PD) [1] and Fast atom bombardment (FAB) [2] mass spectrometry have emerged as powerful tools for the analysis of complex underivatized peptide mixtures obtained from chemical or enzymatic cleaved proteins. It is well known that suppression effects in FAB spectra tryptic are limiting use this technique [3]. This perferential desorption/ionization was mainly attributed to different hydrophobicity individual compounds [4]. In fact, it turned out surface excess concentrations peptides applied liquid matrix were responsible selective behavior [5]. mixture correlated with their average hydrophobicities [4,5] based either on indices Bull Breese [6] Park et al. [7]. severe limitation can be partly reduced by HPLC separation derivatization
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