Prevalence and Characterization of Multi-Drug-Resistant Gram-Negative Bacilli Isolated From Lebanese Poultry: A Nationwide Study.

摘要: Currently, antimicrobial resistance is one of the most prominent public health issues. In fact, there increasing evidence that animals constitute a reservoir resistance. collaboration with Lebanese Ministry Agriculture, aim this study was to determine prevalence intestinal carriage multi-drug-resistant Gram-negative Bacilli in poultry farms at national level. Between August and December 2015, 981 fecal swabs were obtained from 49 distributed across Lebanon. The subcultured on MacConkey agar supplemented cefotaxime (2 μg/ml). Isolated strains identified using MALDI-TOF mass spectrometry. Multilocus sequence typing analysis performed for Escherichia coli. Phenotypic detection extended spectrum β-lactamases (ESBL) AmpC production double disk synergy ampC test, respectively. β-lactamase encoding genes blaCTX-M, blaTEM, blaSHV, blaFOX, blaMOX, blaEBC, blaACC, blaDHA, blaCMY PCR amplification. Out obtained, 203 (20.6%) showed bacterial growth selective medium. Of 235 isolated, 217 as E. coli (92%), eight Klebsiella pneumoniae (3%), three Proteus mirabilis (1%) Enterobacter cloacae (1%). MLST isolates presence ST156, ST5470, ST354, ST155, ST3224. phenotypic tests revealed 43.5, 28.5, 20.5% ampC, ESBL, ampC/ESBL producers, putative TEM gene detected 83% isolates, SHV 20%, CTX-M 53% CMY 65%. Our chicken Lebanon are reservoirs ESBL producing bacilli. level antibiotic consumption veterinary medicine should be evaluated. Future studies focus risk factors associated acquisition organisms farm