Modulation by neurogenic acetylcholine of nitroxidergic nerve function in porcine ciliary arteries.

摘要: Purpose. To determine whether nitroxidergic, cholinergic, and vasoactive intestinal polypeptide (VIP)-mediated nerves participate in the regulation of porcine ciliary arterial tone to analyze mechanisms underlying neuronal interaction. Methods. Changes isometric tension were recorded helical strips arteries, which stimulated by transmurally applied electrical pulses or nicotine. The presence perivascular nerve fibers containing reduced nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase, acetylcholinesterase, VIP immunoreactivity determined histologically. Results. Transmural stimulation (2, 5, 20 Hz) nicotine produced a relaxation denuded endothelium contracted with prostaglandin F2tt. response was not influenced timolol but abolished oxyhemoglobin methylene blue. N°-nitro-L-arginine, nitric oxide (NO) synthase inhibitor, neurogenic relaxation, L-arginine restored response. Physostigmine inhibited, atropine potentiated, attenuated acetylcholine VIP. There bundles NADPH adventitia arteries. Conclusions. Porcine arteries are innervated NO synthase-containing that liberate NO, possibly as neurotransmitter on excitation produce muscular relaxation. Nitroxidergic function is inhibited released from cholinergic nerve, because impaired production release NO. does seem modulator. Invest Ophthalmol Vis Sci. 1997; 38:2261-2269.