Enhanced Biodegradation of Alkane Hydrocarbons and Crude Oil by Mixed Strains and Bacterial Community Analysis

摘要: In this study, two strains, Acinetobacter sp. XM-02 and Pseudomonas XM-01, were isolated from soil samples polluted by crude oil at Bohai offshore. The former one could degrade alkane hydrocarbons (crude diesel, 1:4 (v/v)) efficiently; the latter failed to grow on but produce rhamnolipid (a biosurfactant) with glycerol as sole carbon source. Compared pure culture, mixed culture of strains showed higher capability in degrading which degradation rate increased 89.35 74.32 ± 4.09 97.41 87.29 2.41 %, respectively. grew fast sufficient source produced intermediates subsequently utilized for growth XM-01 then, was XM-01. Till end process, inhibited rapid addition, hydrocarbon 8.06 % compared culture. surface tension medium dropping 73.2 × 10−3 28.6 N/m. Based newly found cooperation between degrader coworking strain, rational investigations optimal strategies biodegradation enhancing biodegradation.