Acute and sub-chronic toxicity of four cytostatic drugs in zebrafish.

摘要: The acute and sub-chronic effects of four cytostatic drugs—5-fluorouracil (5-FU), cisplatin (CisPt), etoposide (ET) imatinib mesylate (IM)—on zebrafish (Danio rerio) were investigated. Acute tests carried out in a static system accordance with the OECD guideline 203 for adult fish draft embryos (FET test) order to find LC50 values drugs. Early-life stage toxicity test on was conducted according 210 using drugs 5-FU IM semistatic objective investigating fish. In fish, ET did not pass limit test, thus, are considered non-toxic. case cisplatin, calculated at 64.5 mg L−1, whereas IM, 70.8 L−1. FET 72-h post fertilization (hpf) 2441.6 CisPt, 349.9 L−1 48 hpf it progressively decreased 81.3 120 hpf. addition, CisPt caused significant delay hatch larvae. ET, calculable as they higher than 300 which concentration substance crystallized solution. hpf; 158.3 , 72 141.6 96 118.0 65.9 Stage Test 5-FU, embryonic deformities detected during tests. Regarding mortalities, 10 can be LOEC, statistically difference mortalities this group alone. Concerning dry body weight standard length, 1 is LOEC. highest tested (10 L−1) LOEC however, treatment have an effect other investigated parameters (dry wet weight, length). All characterized by low