Mitochondrial Ca2+ handling is crucial for generation of rhythmical Ca2+ waves in vascular interstitial cells from rabbit portal vein

摘要: Abstract Vasomotion is the rhythmical changes in vascular tone of various blood vessels. It was proposed that rabbit portal vein (RPV) spontaneous contractile activity driven by interstitial cells (VICs), since RPV VICs generate intracellular Ca2+ concentration ([Ca2+]i) associated with membrane depolarisation these cells. In this work, using confocal imaging Fluo-3 loaded we studied if generation [Ca2+]i affected when handling mitochondria compromised. We also visualised Mito Tracker Green fluorescent dye. Our results showed freshly dispersed generated oscillations a frequency 0.2–0.01 Hz. Imaging stained revealed abundant higher density organelles sub-plasmalemmar region compared to central cell. Oligomycin, an ATP synthase inhibitor, did not affect amplitude and oscillations. contrast, two uncoupling agents, carbonylcyanide-3-chlorophenylhydrazone (CCCP) carbonylcyanide-4-trifluoromethoxyphenylhydrazone (FCCP) effectively abolished simultaneous increase basal VICs. These data suggest mitochondrial important for which underlie vessel.