Efecto de los protocolos de procesamiento sobre la calidad y cantidad de RNA en plasma materno
作者： Liz Ariane , González Ropero
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摘要: Aim: To determine factors affecting the amount of RNA pbsma samples from pregnant women before 20 weeks gestation Materials and Methods: 33 blood weie collected 17 women. Two sample tubes with EDTA were each woman, divided into groups differenis protocols: stored al 4'C or -20;C. without LS Trizol. In addition. extraction was made at drtfemni tin-is .irairti'iml by sperAcphotometry Results: Hwhet levels tfi statisitciiMy significa»! diftoiiva^os iti Ihn sampfos sloiixl ni 4;C win cbtaincxl. wtiilti tini stergo vallatile such as Tii/ol I S Hin lime, did noi afloct Ine concenti ation ol Conclusione: ti pnssibe lo Ktentiry Storage tempsiati;e a vanatte itiot afeets concentratela in materna! plasma.
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