Production of IL 2 and IFN-gamma by T cells from malaria patients in response to Plasmodium falciparum or erythrocyte antigens in vitro.

摘要: T cells from patients acutely infected with malaria exhibit a disease-related stimulation of DNA synthesis in response to Plasmodium falciparum antigen vitro. This is weak and short-lived, suggestive induction suppressor mechanisms. Exogenous cell growth factor (IL 2) that was added antigen-stimulated cultures enhanced proliferation antigen-responsive cultures, indicating the lymphocytes expressed IL 2 receptors. In contrast, addition did not respond had no effect. Antigen-responsive contained endogenous as well, antigen-induced lymphocyte correlated production. However, results suggested production by patients' insufficient or actively shut off, this responsible for premature cessation their synthesis. Supernatants 60% treated 30% RBC ghost interferon-gamma (IFN-gamma), determined cytopathic effect inhibition assay combined acid treatment antibody neutralization an IFN-gamma-specific ELISA. There obvious correlation between presence IFN-gamma culture supernatants. A high activity also seen antigen-treated P. falciparum-immune donors living highly endemic areas. found supernatants healthy vivax malaria. Thus, these appears reflect antigen-reactive may be useful sensitive indicator cellular immunity