Upregulation of miR-582-5p regulates cell proliferation and apoptosis by targeting AKT3 in human endometrial carcinoma

摘要: The human endometrial carcinoma is one of the most common female malignancies, and there an urgent requirement to explore new therapeutic strategies. There accumulating evidence that microRNAs (miRNAs) can serve as potential diagnostic prognostic biomarkers for various types cancer, but significance miR-582-5p still remains largely unknown in carcinoma. aims this study were understand identify influence on proliferation apoptosis its relevant mechanism. First, quantitative real-time PCR (qRT-PCR) was used detect AKT3 expression tissue samples cells. Then, CyQuant assay 2D colony employed evaluate cell proliferation. Western blotting determine protein expression. Subsequently, luciferase reporter target miR-582-5p. Finally, Annexin V apoptosis. We found significantly decreased tissues, upregulation cells inhibit promote Moreover, validated a inversely correlated with Besides, efficiently abrogates effect These results demonstrated regulates via AKT3. Thus, represents meriting further investigation.