Tracking the prokaryotic diversity in acid mine drainage-contaminated environments: A review of molecular methods

摘要: Abstract The negative impact of acid mine drainage (AMD) on public and environmental health by degrading the aquatic terrestrial ecosystem with a drastic decrease in pH elevated levels toxic heavy metals, metalloids radionuclides necessitate development environmentally sustainable technologies to remediate AMD. appropriate strategies for controlling and/or abating detrimental effects AMD natural mining environments primarily depends diversity compositions local acidophilic microbial communities (those which can grow at pH ⩽ 3) catalyze reactions production sulfuric ferric iron. Robust method(s) track AMD-promoting will not only provide information about their ecophysiological role extreme but also help sustain reliability remediation technologies. This paper provides an overview phylogenetic prokaryotes present AMD-impacted environments, different molecular methods that have been used these acidophiles. Additionally, high-throughput (metagenomics, metaproteomics microarrays) link prokaryotic phylogeny function systems are discussed.