HSP70iQ435A-Encoding DNA Repigments Vitiligo Lesions in Sinclair Swine

摘要: Human HSP70iQ435A carries a single amino-acid modification within the dendritic cell activating region and tolerizes cells in vitro. The underlying DNA was used to prevent treat disease in vitiligo mouse models through reduced activation diminished skin T-cell infiltration, suggesting same may be useful for patients. Physiologic differences between human then called studies large animals with human-like skin. We established efficiency of jet injection into swine before subcloning clinically suitable vector pUMVC3. Vitiligo lesions Sinclair were treated plasmid measure changes depigmentation, expression HSP70i skin, serum HSP70i, anti-HSP70i titers. Remarkable repigmentation following HSP70iQ435A-encoding treatment persisted throughout 6-month follow-up period. Repigmentation accompanied by an initial influx T increased CD4/CD8 ratios, waning week 15. Melanocytes spanned border repigmenting that melanocyte repopulation precedes melanization. Serum titer fluctuations not treatment-associated. Importantly, did interfere melanoma immunosurveillance. These data encourage clinical testing HSP70iQ435A.