15N nuclear magnetic resonance of living cells.

摘要: DIRECT observation of nuclear magnetic resonance (NMR) signals in living tissues, cells, or their paniculate components is an appealing aspect NMR spectroscopy as it further extends its applicability from the chemical to biological domain. Of various nuclei available for studies, 1H (refs 1–3), 13C 2 and 4) 31P (ref. 5) have already been observed complex systems. The potential technique study dynamic metabolic processes, or, eventually, aspects cellular physiological changes, has demonstrated6,7. No other affords resolution direct interpretability terms functional groups that provides. One drawback relative insensitivity, especially when representative nuclides interest occur low natural abundance, example, 13C, 15N. In case isotopic enrichment suitably labelled precursors shown provide most valuable data whole cell investigations6. There seem however, be no studies this type using nitrogen NMR. relevance a fundamental element constitutive building blocks lipids, nucleic acids, proteins, even some sugars, suggests observability cells should important probe monitor dynamics control vivo fixation metabolism. We report here results showing 15N-NMR can directly whole, cells.