Movement of sodium into human platelets induced by ADP

摘要: Abstract 1. In normal human platelets the concentrations of Na+ and K+ were 42.1 ± 4.3 98.8 3.7 mequiv/l platelet water respectively (mean S.E. 22 samples). 2. When platelet-rich plasma was incubated with 22Na+ at 37° C for 2–3 h an increase in platelent concentration found which significant after 210 min. Platelet did not change significantly. The radioactivity increased faster than total Na+, suggesting a Nao+-Na+ exchange process unactivated platelets. 3. Addition ADP to resulted aggregation rapid rise (within seconds) 22Na+-radioactivity within 300 s this diminished toward control levels. 4. Under same experimental conditions, bring about 36Cl− 5. Ouabain (10−6 M) added induced platelets, as well decrease concentration. produced further 22Na+, return values, presence ouabain. 6. association but accompanying by suggests selective mechanism movement into rather NaCl together under osmotic gradient.