Identification and functional characterization of four novel aldo/keto reductases in Anabaena sp. PCC 7120 by integrating wet lab with in silico approaches.

摘要: Aldo/keto reductases (AKRs) constitute a multitasking protein family that catalyzes diverse metabolic transformations including detoxification of stress generated reactive aldehydes. Yet this important is poorly understood particularly in cyanobacteria, the ecologically most and significant group micro-organisms. Present study an attempt to characterize all putative AKRs Anabaena sp. PCC 7120. In silico analysis, it revealed presence at least four PCC7120 genome. All proteins share less than 40% sequence identity with each other also identified members AKR superfamily hence deserve be assigned new families. Dissimilarity sequences reflected through their substrate specificity. While reduction trans-2-nonenal, LPO-derived aldehyde was common across proteins, these were found activated during heat, salt, Cd, As, butachlor treatments, ectopic expression Escherichia coli conferred tolerance above abiotic stresses. These findings affirm role providing broad environmental stresses conceivably by detoxifying stress-generated