Isolation, Genomic and Metabolomic Characterization of Streptomyces tendae VITAKN with Quorum Sensing Inhibitory Activity from Southern India.
作者： Nabila Mohammed Ishaque , Ilia Burgsdorf , Jessie James Limlingan Malit , Subhasish Saha , Roberta Teta
DOI: 10.3390/MICROORGANISMS8010121
关键词:
摘要: Streptomyces are among the most promising genera in terms of production ability to biosynthesize a variety bioactive secondary metabolites with pharmaceutical interest. Coinciding increase genomic sequencing these bacteria, mining their genomes for biosynthetic gene clusters (BGCs) has become routine component natural product discovery. Herein, we describe isolation and characterization tendae VITAKN quorum sensing inhibitory (QSI) activity that was isolated from southern coastal part India. The nearly complete genome consists 8,621,231bp GC content 72.2%. Sequence similarity networks BGCs detected this strain against Minimum Information about Biosynthetic Gene Cluster (MIBiG) database 3365 predicted by antiSMASH analysis publicly available were generated through BiG-SCAPE-CORASON platform evaluate its novelty. Crude extract using high-performance liquid chromatography connected high resolution tandem mass spectrometry (HPLC-HRMS/MS) dereplication Global Natural Product Social Molecular Networking (GNPS) online workflow resulted identification cyclic dipeptides (2, 5-diketopiperazines, DKPs) extract, which known possess QSI activity. Our results highlight potential coupled LC-HRMS/MS silico tools as valid approach discovery novel lead compounds. This study also provides diversity an assessment chemical space yet be discovered.
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sci-hub.se 参考文章(51)
Marnix H Medema, Michael A Fischbach, Computational approaches to natural product discovery. Nature Chemical Biology. ,vol. 11, pp. 639- 648 ,(2015) , 10.1038/NCHEMBIO.1884
S. Kodani, M. E. Hudson, M. C. Durrant, M. J. Buttner, J. R. Nodwell, J. M. Willey, The SapB morphogen is a lantibiotic-like peptide derived from the product of the developmental gene ramS in Streptomyces coelicolor Proceedings of the National Academy of Sciences of the United States of America. ,vol. 101, pp. 11448- 11453 ,(2004) , 10.1073/PNAS.0404220101
Adam A. Strömstedt, Jenny Felth, Lars Bohlin, Bioassays in Natural Product Research – Strategies and Methods in the Search for Anti-inflammatory and Antimicrobial Activity Phytochemical Analysis. ,vol. 25, pp. 13- 28 ,(2014) , 10.1002/PCA.2468
Haruo Ikeda, Jun Ishikawa, Akiharu Hanamoto, Mayumi Shinose, Hisashi Kikuchi, Tadayoshi Shiba, Yoshiyuki Sakaki, Masahira Hattori, Satoshi Ōmura, Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis Nature Biotechnology. ,vol. 21, pp. 526- 531 ,(2003) , 10.1038/NBT820
Ari M. Frank, Nuno Bandeira, Zhouxin Shen, Stephen Tanner, Steven P. Briggs, Richard D. Smith, Pavel A. Pevzner, Clustering Millions of Tandem Mass Spectra Journal of Proteome Research. ,vol. 7, pp. 113- 122 ,(2008) , 10.1021/PR070361E
Tomáš Pluskal, Sandra Castillo, Alejandro Villar-Briones, Matej Orešič, MZmine 2: Modular framework for processing, visualizing, and analyzing mass spectrometry-based molecular profile data BMC Bioinformatics. ,vol. 11, pp. 395- 395 ,(2010) , 10.1186/1471-2105-11-395
S. Kumar, K. Kannabiran, Antifungal activity of Streptomyces VITSVK5 spp. against drug resistant Aspergillus clinical isolates from pulmonary tuberculosis patients Journal de Mycologie Médicale. ,vol. 20, pp. 101- 107 ,(2010) , 10.1016/J.MYCMED.2010.04.005
Bin Zhao, Suzy C. Moody, Robert C. Hider, Li Lei, Steven L. Kelly, Michael R. Waterman, David C. Lamb, Structural analysis of cytochrome P450 105N1 involved in the biosynthesis of the zincophore, coelibactin. International Journal of Molecular Sciences. ,vol. 13, pp. 8500- 8513 ,(2012) , 10.3390/IJMS13078500
S. D. Bentley, K. F. Chater, A.-M. Cerdeño-Tárraga, G. L. Challis, N. R. Thomson, K. D. James, D. E. Harris, M. A. Quail, H. Kieser, D. Harper, A. Bateman, S. Brown, G. Chandra, C. W. Chen, M. Collins, A. Cronin, A. Fraser, A. Goble, J. Hidalgo, T. Hornsby, S. Howarth, C.-H. Huang, T. Kieser, L. Larke, L. Murphy, K. Oliver, S. O'Neil, E. Rabbinowitsch, M.-A. Rajandream, K. Rutherford, S. Rutter, K. Seeger, D. Saunders, S. Sharp, R. Squares, S. Squares, K. Taylor, T. Warren, A. Wietzorrek, J. Woodward, B. G. Barrell, J. Parkhill, D. A. Hopwood, Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2) Nature. ,vol. 417, pp. 141- 147 ,(2002) , 10.1038/417141A
Jennifer Campbell, Qi Lin, Grant D. Geske, Helen E. Blackwell, New and unexpected insights into the modulation of LuxR-type quorum sensing by cyclic dipeptides. ACS Chemical Biology. ,vol. 4, pp. 1051- 1059 ,(2009) , 10.1021/CB900165Y