Involvement of the Fas System in Hepatitis C Virus Recurrence After Liver Transplantation

摘要: To date, there have been no reports of the involvement Fas system in recurrent hepatitis C virus (HCV) infection after orthotopic liver transplantation (OLT). In 25 patients who underwent OLT for HCV-related cirrhosis, we evaluated expression antigen (FasAg) on hepatocytes, apoptic and serum levels soluble (sFas). The level HCV viremia genotype were determined by polymerase chain reaction. Serum sFas an enzyme immunoassay procedure. DNA fragmentation was terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end-labeling (TUNEL) technique deparaffinized samples. FasAg immunoperoxidase method. Sixteen had evidence disease. number hepatocytes expressing percentage apoptotic greater among developed than those did not (P < .01 P .0001, respectively). There a correlation between hepatic FasAg, intensity lobular inflammation = .007), TUNEL index .001). with without .04). We conclude that (1) is up-regulated related to grading disease; likewise, hepatitis; (2) demonstration labeling nuclei assay suggest apoptosis mediated may role pathogenesis OLT. (Liver Transpl 2000;6:562-569.)