Conversion of (3S,4R)-Tetrahydrodaidzein to (3S)-Equol by THD Reductase: Proposed Mechanism Involving a Radical Intermediate
作者： Mihyang Kim , E. Neil G. Marsh , Soo-Un Kim , Jaehong Han
DOI: 10.1021/BI100465Y
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摘要: To elucidate the mechanism of (3S)-equol biosynthesis, (2,3,4-d3)-trans-THD was synthesized and converted to by THD reductase in Eggerthella strain Julong 732. The position deuterium atoms determined 1H NMR 2H spectroscopy, product identified as (2,3,4α-d3)-(3S)-equol. All were retained, while OH group at C-4 replaced a hydrogen atom with retention configuration. explain this stereospecific reduction, we propose involving radical intermediates.
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