Genotoxic effect of bile acids on human normal and tumour colon cells and protection by dietary antioxidants and butyrate.

摘要: Colorectal cancer is the second cause of death for tumour worldwide. Among risk factors this disease dietary habits seem to have a pivotal role. An elevated intake fats causes high release in gut lumen bile acids that are positively correlated with colorectal cancer, since they act as detergents and proliferation promoters. Recently, it was evidenced can also be able induce DNA damage. In study genotoxicity deoxycholic acid (DCA) chenodeoxycholic CDCA) has been evaluated human normal colonocytes derived from 60 colon biopsies cells. The involvement reactive oxygen species (ROS) oxidative damage assessed. addition, protective effect exerted by both two well-known antioxidants commonly present diet, β-carotene α-tocopherol, butyrate which known involved regulation several cellular functions, tested. “comet assay” or single cell gel electrophoresis (SCGE) its conventional use Endonuclease III modified method, allow detect presence oxidized pyrimidines. Bile (CDA resulted genotoxic on inclusion endonuclease digestion step comet assay demonstrated induced an treatment (β-carotene, α-tocopherol) Na-butyrate caused reduction Our results suggest may initiation inducing damage, so add further evidences preventive properties Mediterranean diet.