Monoclonal Antibody Directed Against Heparin and Heparin-Fractions

作者: Günter Huhle , Job Harenberg , Reinhard Malsch , Dieter L. Heene

DOI: 10.1007/978-1-4613-0371-8_4

关键词:

摘要: A monoclonal heparin antibody H 1.18 has been raised against native using a heparin-bovine serum albumin conjugate prepared by reductive amination. The was purified protein A/G affinity chromatography. For further analyses tyramine, which covalently bound to low molecular mass endpoint attachment (Maisch R et al: Anal Biochem 1994; 217: 255–264), labeled with 125-iodine at the aryl residue. tracer complex immunoprecipitated goat anti-mouse immunoglobuline IgG. recognized specifically intact and fractions. lower detection limit of preparations 100 ng/ml. No cross reactivity occurred other glycosaminoglycans such as heparan sulfate, dermatan chondroitin sulfate C. Oversulfated showed than 2–0-and 6–0-desulfated heparin.

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