Intracellular dynamics of polydnavirus innexin homologues.

摘要: Polydnaviruses associated with ichneumonid parasitoid wasps (Ichnoviruses) encode large numbers of genes, often in multigene families. The Ichnovirus Vinnexin gene family, which is expressed parasitized lepidopteran larvae, encodes homologues Innexins, the structural components insect gap junctions. Here, we have examined intracellular behaviours Campoletis sonorensis (CsIV) Vinnexins, alone and combination a host Innexin orthologue, Innexin2 (Inx2). QRT-PCR verified that transcription CsIV vinnexins occurs contemporaneously inx2, implying co-occurrence Inx2 proteins. Confocal microscopy demonstrated epitope-tagged VinnexinG (VnxG) VinnexinQ2 (VnxQ2) exhibit similar subcellular localization as Spodoptera frugiperda (Sf-Inx2). Surface biotinylation assays all three proteins localize to cell surface, cytochalasin B nocodazole they rely on actin microtubule cytoskeletal networks for localization. Immunomicroscopy following co-transfection constructs indicates extensive co-localization Vinnexins each other Sf-Inx2, live-cell imaging mCherry-labelled supports may affect Sf-Inx2 distribution Vinnexin-specific fashion. Our findings support disrupt physiology protein-specific manner through altering junctional intercellular channel communication, well indirectly by affecting multicellular junction characteristics.