The spatial expression patterns of a phosphate transporter (MtPT1) from Medicago truncatula indicate a role in phosphate transport at the root/soil interface

摘要: Summary The movement of phosphate from the soil into plant root cells is first many crucial transport events required to supply phosphorous (P) throughout plant. In addition ability acquire soil, majority vascular plants are able form arbuscular mycorrhizal associations in which may be delivered cortex via a fungus. Previously, we cloned two transporter genes, MtPT1 and MtPT2 Medicago truncatula roots. Complementation yeast mutant revealed that functional Northern analyses expressed exclusively roots (Liu et al., 1998, Mol. Plant-Microbe Interact. 11, 14–22). Utilising an antibody specific for MtPT1, have analysed accumulation spatial expression patterns transporter. transcript protein levels show close correlation increase dramatically response starvation. decrease during development symbiosis with (AM) fungi, indicating this not involved symbiotic transport. Membrane fractionation analysis MtPT1/GFP fusion located plasma membrane, while situ hybridisation immunolocalisation demonstrate presence transcripts epidermal hairs M. shows consistent role specifically acquisition distinct other class described date.