The Effect of Pancreas Islet-Releasing Factors on the Direction of Embryonic Stem Cells Towards Pdx1 Expressing Cells

摘要: Diabetes mellitus, which is the result of autoimmune destruction insulin-producing β cells, occurs by loss insulin-secreting capacity. The insufficient source cells (IPCs) major obstacle for using transplantation as diabetes treatment method. present study suggests a method to form islet-like clusters IPCs derived from mouse embryonic stem (mESCs). This protocol consists several steps. Before starting this protocol, embryoid bodies (EBs) should be cultured in suspension conditioned medium isolated pancreatic islet combination with activing A induced. Then differentiated mESCs were replaced dishes supplemented basic fibroblast growth factor (bFGF). Next, bFGF was withdrawn, and cyclopamine noggin added. treated B27, nicotinamide, islet-conditioned maturation. mESCs, control group, without any treatment. An enhanced expression pancreatic-specific genes detected qRT-PCR immunofluorescence mESCs. mESCsco express other markers well insulin. exhibited higher insulin generation further improvement that may an unlimited ES suitable transplantation. results indicated medium, just critical components cell niche associated factors, had high potential differentiate into IPCs.