Culture of pig embryos

摘要: Pig embryos can be cultured using a number of different strategies including complex approaches like culture in vivo surrogate oviduct (rabbit, sheep, mouse), mouse oviducts organ culture, and co-culture with cells addition to simple defined media or salt solutions. Addition serum medium has been particular importance where blastocyst development hatching are required. conceptuses (day 10-15), embryonic discs cell lines derived from Eagle's minimal essential Dulbecco's modified serum, although the absence serum. In contrast, early stage pig (one-cell blastocyst) best simpler such as those used for embryos. The that have all relatively similar composition. They contain salts one more energy sources glucose, lactate, pyruvate BSA macromolecular component. Early attempts were not very successful, but some did develop stage. More recent reports indicate much higher rate little no change Some workers reported improved lacking which is consistent findings laboratory animals hamsters. Glutamine serve sole exogenous source lactate pyruvate. taurine hypotaurine enhances vitro. We suggest, possible, adoption standard could by laboratories and, perhaps, species. Use species would simplify experimental protocols, enhance studies comparative physiology metabolism, expedite transfer information obtained