Proteomics-based investigation of multiple stages of OSCC development indicates that the inhibition of Trx-1 delays oral malignant transformation.

摘要: The majority of cases oral squamous cell carcinoma (OSCC) develop from potentially malignant disorders, which have been confirmed to be involved in chronic oxidative stimulation. However, no effective treatment approaches used prevent the development dysplasia into cancerous lesions thus far. In present study, a well-established OSCC model was detect proteomics profiles at different stages during transformation. Of 15 proteins that were found upregulated both and stages, stress-associated proteins, thioredoxin-1 (Trx-1), glutaredoxin-1 peroxiredoxin-2 note as with significant changes expression Trx-1 identified most significantly protein precancerous stage. Validation experiments overexpressed tissue samples, inhibition able promote apoptosis cells under hypoxic conditions. Furthermore, experimental application Trx-1-specific inhibitory agent an animal led lower cancerization rate delay tumor formation. possible mechanisms associated increased via reactive oxygen species (ROS)-dependent pathway. Taken together, our findings indicate may important target for delaying transformation, provides novel therapeutic strategy prevention OSCC.