Tyrosine hydroxylase phosphorylation in catecholaminergic brain regions: a marker of activation following acute hypotension and glucoprivation.

摘要: The expression of c-Fos defines brain regions activated by the stressors hypotension and glucoprivation however, whether this identifies all sites involved is unknown. Furthermore, neurochemicals that delineate these regions, or are utilized in them when responding to remain undefined. Conscious rats were subjected hypotension, vehicle for 30, 60 120 min changes phosphorylation serine residues 19, 31 40 biosynthetic enzyme, tyrosine hydroxylase (TH), activity TH and/or, determined, up ten simultaneously contain catecholaminergic cell bodies and/or terminals: A1, A2, caudal C1, rostral A6, A8/9, A10, nucleus accumbens, dorsal striatum medial prefrontal cortex. Glucoprivation evoked C1 accumbens whereas A10 cortex 30 post stimulus few evident at min. Although increases pSer19, indicative depolarization, seen where was evoked, a sensitive measure activation A8/9 did not express contains only terminals. Specific patterns residue detected, dependent upon region, suggesting distinct signaling cascades. Hypotension reduction A1 suggestive reduced kinase activity. increased, indicating synthesis TH, pSer31 alone increased (prefrontal cortex) conjunction with pSer40 (caudal C1). Thus, provide highly activity, cellular catecholamine utilization short term, response glucoprivation.