SDS‐PAGE: a tool to discriminate Xylella fastidiosa from other endophytic grapevine bacteria*

摘要: There is a real risk of introducing Xylella fastidiosa into the EPPO region where this quarantine pest has not yet been found and constitutes serious threat to Mediterranean grapevine industry. Direct isolation endophytic bacteria from imported propagation material, combined with one-dimensional SDS-PAGE total soluble proteins bacterial cell envelope, can be considered as test used in certification schemes. The gel electrophoretic pattern characterized by constant distribution 14 major diagnostic bands. Even without scanning, relatively simple technique allows discrimination between strains X. other xylem-inhabiting, slow-growing bacteria.