DNA-templated formation and luminescence of diphenylacetylene dimeric and trimeric complexes.

摘要: We report on spectral features for two and three diphenylacetylene chromophores aligned in close proximity aqueous solution by self assembly of attached oligonucleotide arms. Two duplex systems were examined detail. One was formed hybridization (Watson–Crick base pairing) 10-mers, each containing the insert. The other generated self-folding a 36-mer inserts. triplex system obtained (Hoogsteen 16-mer conjugate to folded hairpin. Formation entities from these conjugates demonstrated experimentally thermal dissociation spectroscopic studies. UV CD spectra exhibit bands 300–350 nm region attributable exciton coupling between chromophores, emission show strong band centered at 410 assigned excimer fluorescence. Addition third strand hairpin has little effect spectrum region, but leads negative short wavelengths characteristic strongly enhanced fluorescence spectrum. alone shows broad ∼345–365 nm, this is virtually absent system. A model proposed which diphenylacetylenes function as ground state dimer that excitation gives rise observed Excitation chromophore results fluorescence, consequence energy transfer locally excited singlet one chromophores.