Identification, characterisation and molecular modelling of two AP endonucleases from base excision repair pathway in sugarcane provide insights on the early evolution of green plants.

摘要: Unlike bacteria and mammals, plant DNA repair pathways are not well characterised, especially in monocots. The understanding of these processes the cell is major importance, since they may be directly involved acclimation adaptation to stressful environments. Hence, two sugarcane ESTs were identified as homologues AP endonuclease from base-excision pathway: ScARP1 ScARP3. In order understand their probable function evolutionary origin, structural phylogenetic studies performed using bioinformatics approaches. predicted proteins present a considerable amino acid sequence similarity, molecular modelling procedures indicate that both functional, main motifs remain conserved. However, inspection sort signal regions on full-length cDNAs indicated have distinct organelle target. Furthermore, variances promoter cis-element also found. Although mRNA expression pattern was similar, there significant differences levels. Taken together, data raise hypothesis ScARP an example gene duplication event occurred before monocotyledon/dicotyledon segregation, followed by sub-functionalisation Poaceae, leading new intracellular targeting different