Cannabinoids in pancreatic cancer: correlation with survival and pain.

摘要: Cannabinoids exert antiproliferative properties in a variety of malignant tumors, including pancreatic ductal adenocarcinoma (PDAC). In our study, we quantitatively evaluated the immunoreactivity for cannabinoid-1 (CB1) and cannabinoid-2 (CB2) receptors as well endocannabinoid metabolizing enzymes fatty acid amide hydrolase (FAAH) monoacyl glycerol lipase (MGLL). Furthermore, quantitative real-time RT-PCR CB1, CB2, FAAH MGLL normal pancreas cancer tissues was performed. Levels endocannabinoids were determined by liquid chromatography/mass spectrometry. Immunoreactivity scores QRT-PCR expression levels correlated with clinico-pathological (TNM, survival, pain) status patients. Evaluation revealed that these remained unchanged PDAC compared to pancreas. Patients high CB1 receptor enlarged nerves had lower combined pain score (intensity, frequency, duration; p = 0.012). There significant relationship between low or mRNA (p 0.0011 0.026, respectively), cell 0.036 0.017, respectively) longer survival These results are underlined correlation increased 0.0343). CB2 immunoreactivity, receptor, did not correlate survival. Therefore, changes cannabinoid on cells may affect prognosis