Changes of membrane proteins and their relation to deoxyribonucleic acid synthesis and cell division of Escherichia coli.

摘要: Abstract Escherichia coli K-12 were treated in five ways to alter DNA synthesis, cell division, or both. Their membranes then examined for changes. The bacteria grown the presence of radioactive l-arginine label membranes. 14C-Arginine was used experimental culture and 3H-arginine control, vice versa. cells mixed, their isolated run on acrylamide gels 0.5% sodium dodecyl sulfate obtain a spectrum proteins. Gel fractions counted both labels order find differences caused by treatment. Two principal changes found At position Y, corresponding molecular weight 34,000, less protein appeared whenever synthesis inhibited. X, 39,000, extra bacterial division Rates Y X during thymidine deprivation stop after restoration thymidine. Whereas rapidly affected, gradual, suggesting that is not responsible DNA; rather, its slowed rate formation might be consequence arrested synthesis. Changes more immediate, could possibly altered which only affected lag. No significant noted between near beginning end cycle, these proteins are normally made continuously produced under normal conditions. Pulse chase experiments indicated do have precursor-product relation. This also suggested kinetic studies. Temperature-sensitive membrane previously described temperature-sensitive mutant further examined. mutation appears influence indirectly stopping But production absence much than wild type, compatible with ability divide even