Harvesting recombinant microbial cells using crossflow filtration
作者： F.James Bailey , R.Thomas Warf , Robert Z. Maigetter
DOI: 10.1016/0141-0229(90)90002-8
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摘要: Abstract A contained, crossflow filtration (CFF) membrane system is described for harvesting Saccharomyces cerevisiae and Escherichia coli cells. This portable can be cleaned sanitized in place. Low- high-cell density (LCD, HCD) fermentations of recombinant cells 10- to 200-l volumes were used as the starting material. LCD fermentations, up 8.3 g l−1 dry weight (dcw) S. cerevisiae, with 10 200 l harvested diafiltered 0.5 1.5 h, respectively. HCD (47–63 dcw) approximately 2 h. E. (up 16.2 dcw), 2.3 h while employing 14 75 ft2 area, Using hollow fiber or flat sheet membranes from different sources, cell times less than 2.5 These studies demonstrate that CFF an efficient method diafiltering fermentation broth.
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