Highly sensitive determinaiton of maltooligosaccharides by HPLC with Chemiluminescence
作者： Tomokazu KATO , Kimihiko ABE , Kazumi NAKAMURA , Masaki TACHIBANA , Hitoshi KOIZUMI
DOI: 10.2116/BUNSEKIKAGAKU.52.741
关键词:
摘要: グルコース（G1）及びマルトオリゴ糖の混合物（G2～G7）を，McIlvaine緩衝溶液（pH 5.0）を移動相としてC30固定相を用いた逆相液体クロマトグラフ法により分離し，溶出した各糖を固定化アミログルコシダーゼカラムリアクターによりグルコースに加水分解し，固定化ピラノース酸化酵素カラムリアクターにより酸化し，生成した過酸化水素を固定化ペルオキシダーゼ充填フローセルでルミノール化学発光検出した．C30固定相によって，G3～G7のアノマー分離が生じた．検出限界（S/N＝3）（注入量20 μl）は，G1，G2，G3，G4，G5，G6，G7でそれぞれ8，8，8，10，10，300，500 nM（G7）であった．本法を市販清涼飲料水中の糖分析へ応用した．
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