Effect of growth factors on nuclear and mitochondrial ADP-ribosylation processes during astroglial cell development and aging in culture

摘要: Epidermal growth factor (EGF), basic fibroblast (bFGF), insulin-like factor-I (IGF-I) and insulin (INS) are powerful mitogens may regulate gene expression in cultured astrocytes by ADP-ribosylation process. Nuclear poly-ADP ribose polymerase (PARP) mitochondrial monoADP-ribosyltransferase (ADPRT) the key enzymes involved poly-ADP-ribosylation mono ADP-ribosylation, respectively. In this investigation effect of EGF, bFGF, IGF-I or INS on nuclear PARP ADPRT activities were assessed nuclei mitochondria purified from developing (30 DIV) aging (90 190 primary rat astrocyte cultures. A marked increase activity bFGF treated astroglial cell cultures at 30 DIV was found. greatly stimulated treatment with EGF alone together DIV. showed a more remarkable control untreated than 90 These findings suggest that process is DNA damage repair during differentiation culture. Twelve hours significantly The above results indicate play crucial role post-translational modification chromosomal proteins including vitro models. This suggests factors genomic stability glial cells development maturation, stimulating processes