Evolution of Culture Vessel for Micropropagation: From Test Tube To Culture Room

摘要: To improve the culture conditions for micropropagation, different types of vessels and capping systems have been designed. Some these designs aerial composition in vessel some recycling nutrient medium. This article describes evolution systems, with special emphasis on forced ventilation to atmosphere thus growth multiplication also quality propagules. By altering environment vessel, plantlets can be grown photoautotrophically (sugar free medium) which has many advantages over photomixotrophic or heterotrophic system. using a photoautotrophic system, scaling-up is possible high rate survival percentage minimum time space. More recently, this scale-up system further extended making aseptic room itself large containing small sterile trays plants shelves common headspace. enlarged production even more transplants was achieved relatively easily.