Activation and Inhibition in the Phosphoglucomutase System

摘要: The activation of phosphoglucomutase prior to the enzymic assay is interpreted in terms direct removal inhibiting metals or their replacement by Mg2+ which occurs readily only absence presence very low concentrations substrate. Inhibition enzyme high caused formation inactive magnesium complexes glucose 1-phosphate and 1,6-diphosphate complicated dead-end substrate inhibition. chloride, acetate, bromide, nitrate, iodide, perchlorate increases order listed. pKaa3 pKaa4 1,6-diphosphoric acid are 6.0 6.8, respectively, at ionic strength 0.024, thus ruling out possibility a stable hydrogen bond between two phosphate groups trianion. Comparison binding glucose-1-P glucose-1,6-di-P indicates that bidentate effects involving unimportant.