Field evaluation of transgenic potato plants expressing an antisense granule-bound starch synthase gene: increase of the antisense effect during tuber growth.
作者： Anja G. J. Kuipers , Wim J. J. Soppe , Evert Jacobsen , Richard G. F. Visser
DOI: 10.1007/BF00019490
关键词:
摘要: Transgenic plants of a tetraploid potato cultivar were obtained in which the amylose content tuber starch was reduced via antisense RNA-mediated inhibition expression gene encoding granule-bound synthase (GBSS). GBSS is one key enzymes biosynthesis and catalyses formation amylose. The genes, based on full-length cDNA driven by 35S CaMV promoter or promoter, introduced into genome Agrobacterium tumefaciens-mediated transformation. Expression each these genes resulted complete expression, thus production amylose-free starch, mature field-grown originating from rooted vitro plantlets 4 out 66 transgenic clones. Clones incompletely inhibited showed an increase extent during growth. This likely to be due granule size growth specific distribution pattern components granules clones with activity. higher stability tubers as compared promoter. Field analysis indicated that could achieved without significantly affecting sugar tubers, level other involved metabolism agronomic characteristics such yield dry matter content.
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