The effects of lead and cadmium on GATA-1 regulated erythroid gene expression.

摘要: Lead (Pb) and cadmium (Cd) are heavy metal toxins that cause many pathophysiologic effects, including anemia. Previous in vitro studies have shown these metals able to replace coordinated Zinc (Zn) atoms the Zn fingers of transcription factors this can alter structure DNA-binding characteristics proteins. This has lead hypothesis one mechanism underlying toxic effects Pb Cd is their ability finger factor function resulting aberrant target gene expression. A recent report hematopoietic GATA-1 prompted us address setting MEL cell differentiation. If or inhibit function, should be detectable through alterations chemically induced erythroid differentiation GATA-1-dependent Despite a strong rationale for hypothesis, we found no significant change differentiation, expression several genes, vivo binding DNA at concentrations well above those associated with humans. These results argue against significantly alters vivo.