Basic fibroblast growth factor stimulates cytosolic phospholipase A2, phospholipase C-gamma1 and phospholipase D through distinguishable signaling mechanisms.

摘要: Fibroblast growth factors (FGFs) stimulate proliferation, differentiation and motility of different cell types. The cellular effects FGF are transduced by its interaction with any one four members a family high affinity, surface receptors (FGFRs) that have autophosphorylating tyrosine kinase activity. Activation FGFR causes release various low molecular weight signaling molecules which required for the pleotropic FGFs. We report here basic plays critical role in membrane phospholipid hydrolysis NIH 3T3 cells stably transfected FGFR1. Upon binding to FGFR1, stimulates cytosolic form phospholipase A2 (cPLA2), C-gamma1 (PLC-gamma1) D (PLD), key enzymes production lipid second messengers, kinase-dependent manner. In addition phosphorylation, cPLA2 catalytic activation requires serine phosphorylation p42 mitogen-activated protein (MAP) possibly pertussis toxin-sensitive G-protein coupling. On other hand, phosphatidyl inositol 4,5 bisphosphate (PIP2) direct at residue PLC-gamma1 isozyme. PLD needs or indirect receptor C (PKC) activities. Additionally, it also botulinum toxin C-sensitive Rho-like activation. All these results suggest exerted through individual effectors activated via distinguishable mechanisms according cell's need.