Purification of a highly modified RNA-aptamer Effect of complete denaturation during chromatography on product recovery and specific activity
作者： Philippe Bridonneau , Steve Bunch , Robert Tengler , Ken Hill , Jeff Carter
DOI: 10.1016/S0378-4347(99)00037-7
关键词:
摘要: To evaluate RNA-aptamers as potential drug candidates, efficient and scaleable purification protocols are needed. Because aptamers highly structured rigid molecules, denaturation during the process is a critical aspect to obtain pure active product. A two-step chromatographic procedure was developed purify synthetic anti-VEGF aptamer at preparative scale. reversed-phase step optimized with hydrophobic ion pairing reagent, followed by ion-exchange chromatography in which heat chaotropic salt were used. of presence 2'-modified ribose, conditions had be both steps achieve fully molecule.
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