Elucidation of the genetic and epigenetic landscape alterations in RNA binding proteins in glioblastoma

摘要: // Shruti Bhargava 1, * , Vikas Patil 2, Kulandaivelu Mahalingam 2 Kumaravel Somasundaram 1 Department of Microbiology and Cell Biology, Indian Institute Science, Bangalore-560012, India Bio-Medical Sciences, School Biosciences Technology, VIT University, Vellore-632014, These authors have contributed equally to this work Correspondence to: Somasundaram, email: skumar@mcbl.iisc.ernet.in Keywords: glioblastoma, RNA binding proteins, regulation, signature, cancer stem cells Received: April 15, 2016     Accepted: November 30, Published: December 27, 2016 ABSTRACT proteins (RBPs) been implicated in development. An integrated bioinformatics analysis RBPs (n = 1756) various datasets 11) revealed several genetic epigenetically altered events among glioblastoma (GBM). We identified 13 mutated 472 differentially regulated GBM samples. Mutations AHNAK predicted poor prognosis. Copy number variation (CNV), DNA methylation miRNA targeting RBP differential regulation. Two sets that may be initial astrocytic transformation glioma progression were identified. also a four (NOL3, SUCLG1, HERC5 AFF3) having unique expression pattern stem-like (GSCs), an independent prognostic indicator GBM. risk score derived from the signature stratified into low-risk high-risk groups with significant survival difference. Silencing NOL3, SUCLG1 inhibited GSC maintenance. Gene set enrichment genes between underscored importance inflammation, EMT hypoxia Thus, we provide comprehensive overview epigenetic regulation development progression.