Temporal expression of IL-1β protein and mRNA in the brain after systemic LPS injection is affected by age and estrogen

摘要: Abstract Estrogen has been shown to suppress neural inflammation in vivo response intracerebral LPS injections or by intraparenchymal of NMDA. Using the latter approach, we have that estrogen suppresses inflammatory cytokine expression lesioned ovariectomized young adult females but not reproductive senescent animals. However, cultured microglia derived from either animals, fails LPS-induced expression. These data suggest estrogen's effects on may result its actions blood-borne immune cells at blood brain barrier both. This hypothesis was directly tested here using a systemic injury model and comparing olfactory bulb, which is protected barrier, pituitary gland, incompletely barrier. Young Sprague–Dawley female rats were replaced with an placebo pellet. Three weeks later, animals received single i.p. injection (or vehicle) terminated 0.5, 2 3 h later. Systemic increased IL-1β liver time-dependent manner females. In adults, both bulb gland. treatment attenuated completely suppressed while placebo-replaced responded normally. age-related difference induction also seen mRNA regulation, such induced adults female. Age hormone cytokines mirrored plasma corticosterone (CORT) levels, CORT. central insult can be modulated although mechanism underlying initiation this varies age.