Malignant B lymphocytes from non-Hodgkin's lymphoma induce allogeneic proliferative and cytotoxic T cell responses in primary mixed lymphocyte cultures: An important role of co-stimulatory molecules CD80 (B7-1) and CD86 (B7-2) in stimulation by tumor cells

摘要: We analyzed the stimulating capacities of malignant B cells from non-Hodgkin's lymphomas (NHL) to induce an allogeneic response in primary mixed lymphocyte reaction (MLR). T purified a single healthy donor (KS) were used compare responses induced by either or hyperplastic cells. Malignant strong proliferation KS independently their level expression adhesion molecules. The after MLR predominantly CD3+, CD25+, HLA-DR+, Ki67+ and CD45RO+ cells, CD4/CD8 ratio was heterogeneous (from 0.8 2.7). To investigate role co-stimulatory molecules CD80 CD86 for stimulatory both before during MLR. Most fresh negative CD86, whereas co-cultured expressed high levels This crucial cell proliferation, since monoclonal antibodies directed against completely abrogated also report that responding at end co-culture able lyse as stimulator different extents 10 51%), lysis enhanced PMA activation target Inhibition experiments using CD8 CD4 mAb showed effector mainly CD8+. is first describe accessory function human NHL sensitivity mediated CD8+ suggests new strategies development antitumor immunity NHL.