Molecular and genomic studies of temperate phages from Halomonas aquamarina and Bacillus spp. isolates from the Gulf of Mexico

摘要: Viruses are the most abundant biological entities in ocean and believed to contribute nutrient cycling, bacterial diversity, horizontal gene exchange. However, little is known about relationship between temperate phages their hosts marine environments. In this thesis, phage-host systems from Gulf of Mexico were used study influence bacteria. ФHAP-1 a myovirus induced with mitomycin C Halomonas aquamarina isolate. The genome phage was 39,245 nucleotides long contained 46 predicted genes. Besides genes involved lysogeny, protelomerase, which responsible for resolution telomeric ends linear plasmid-like phages. Hybridization studies PCR analysis indicated not only lack integration prophage host chromosome, but differences arrangement virion forms ФHAP-1. These results suggest that exists as nonintegrating ends. Eleven pigmented Bacillus spp. isolates examined occurrence lysogeny sporulation through induction decoyinine, viii respectively. these experiments suggested variety interactions can occur hosts, some may sporulation. lysogenic strain B14905 had high frequency selected further analysis. 4 prophage-like regions, one independently sequenced an lysate. TEM lysate two regions inducible prophage, defective phage, non-inducible remnant. One prophages transcriptional regulator hypothesized be regulation diversity elements found genetic oceans vast. H. illustrates molecular, genomic, function provide insight into on