Protein kinase A phosphorylation and G protein regulation of purified renal Na+ channels in planar bilayer membranes.
作者： D J Benos , I I Ismailov , J H McDuffie
DOI: 10.1016/S0021-9258(17)34052-8
关键词:
摘要: Purified bovine renal epithelial Na+ channels incorporated into planar lipid bilayer membranes were used to evaluate the biophysical consequences of its phosphorylation by protein kinase A (PKA). We also studied effects pertussis toxin-induced ADP-ribosylation on single channel activity nonphosphorylated and PKA-phosphorylated channels. PKA-induced resulted in a significant increase open probability (Po) with no change conductance, as well increased multiple openings bilayer. Further, PKA conferred voltage sensitivity gating without affecting conduction properties. inhibited subsequent toxin (PTX). Addition guanosine 5'-3-O-(thio)triphosphate reversed this inhibition. However, exposure PTX factor 3-5. These results demonstrate that cAMP-dependent pathway is an important regulatory element for amiloride-sensitive PTX-induced channel-associated Gi function depend upon previous state protein.
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